Fecha de publicación: -- Fuente: PubMed "royal jelly" Food Res Int. 2026 Dec 1;245(Pt 4):120857. doi: 10.1016/j.foodres.2026.120857. Epub 2026 Sep 28.ABSTRACTAssessing the quality and authenticity of royal jelly (RJ) is essential due to its high nutritional and commercial value. In this study, Greek and Chinese RJ samples were analyzed using Liquid Chromatography coupled to Trapped Ion Mobility Spectrometry and High-Resolution Mass Spectrometry (LC-TIMS-HRMS) and compared for the first time to investigate their metabolomic profile. A total of 21 bioactive compounds from different classes were identified through target screening, while quantitative differences between the two were revealed. Chemometric evaluation contributed to the detection of characteristic compounds like quercetin, luteolin, and pinocembrin for the Greek geographical origin. In addition, the combination of non-target screening with chemometrics achieved 100% classification accuracy between Greek and Chinese samples and enabled the identification of additional discriminatory features, such as pentadecanoic acid, tetradecanoic acid, and oleic acid. Furthermore, non-target screening methodology was employed to assess the adulteration of Greek RJ with Chinese RJ, successfully detecting adulteration levels as low as 1%. Overall, the proposed LC-HRMS-based workflow can be an effective approach for the simultaneous authentication and detection of adulteration of RJ.PMID:42967694 | DOI:10.1016/j.foodres.2026.120857