Fecha de publicación: -- Fuente: PubMed "propolis" J Oral Biosci. 2026 Nov 2;68(6):100829. doi: 10.1016/j.job.2026.100829. Online ahead of print.ABSTRACTOBJECTIVES: To produce and phytochemically evaluate hydroalcoholic extracts of green propolis (HEGP) and pomegranate peel (HEPP), and to evaluate their antimicrobial and antibiofilm activities, individually and in combination, against Acinetobacter baumannii and Pseudomonas aeruginosa. Additionally, the cytotoxicity of these extracts was evaluated using the human keratinocyte cell line, HaCaT.METHODS: The extracts were prepared and characterized by high-performance liquid chromatography. Antimicrobial activity was determined using the broth microdilution method (CLSI - M7-A9), to establish the minimum bactericidal concentration (MBC). The checkerboard technique was used to analyze extract combinations, adopting the Fractional Bactericidal Concentration Index. Synergistic and additive concentrations were further tested on monotypic biofilms using the MTT assay. Statistical analysis was performed using analysis of variance and Tukey's test.RESULTS: Chromatographic analysis revealed a punicalagin peak in HEPP and an artepillin C peak in HEGP. Soluble solids content was 60.2 mg/mL for HEPP and 80.4 mg/mL for HEGP. Both extracts had bactericidal activity, with HEPP MBC values ranging from 1 mg/mL to 15 mg/mL and HEPP MBPC values ranging from 2 mg/mL to 20.1 mg/mL. Combined extracts had additive and synergistic effects, and they had antibiofilm activity comparable to that of chlorhexidine digluconate. There were no cytotoxic effects in HaCaT cells.CONCLUSIONS: HEPG and HEPP had antimicrobial and antibiofilm action against P. aeruginosa and A. baumannii, they had good antioxidant action, and they were not cytotoxic to HaCaT cells.PMID:42970941 | DOI:10.1016/j.job.2026.100829