Fecha de publicación: -- Fuente: PubMed "propolis" Biogerontology. 2026 Nov 6;27(6):192. doi: 10.1007/s10522-026-10539-5.ABSTRACTAging is accompanied by alterations in inflammatory signaling, regulated cell-death pathways, and cellular stress responses in the brain. This study investigated the effects of propolis and dexpanthenol, administered individually or in combination, on gasdermin-related, inflammatory, and heat-shock gene expression in naturally aged male mice. Thirty-five male CD-1 mice were randomly allocated to five groups (n = 7/group): Young Control, Aged Control, Aged + Propolis, Aged + Dexpanthenol, and Aged + Propolis + Dexpanthenol. Propolis (200 mg/kg/day) and dexpanthenol (500 mg/kg/day) were administered for 14 days. Whole-brain mRNA expression of GSDMD, GSDME, GSDMC, TNF-α, IL-1β, HSP70, and HSP90 was evaluated by quantitative real-time PCR. Natural aging was associated with marked reductions in TNF-α, IL-1β, and HSP70 expression, whereas GSDMD, GSDME, GSDMC, and HSP90 were not significantly altered after correction for multiple comparisons. Propolis reduced GSDME and IL-1β expression while increasing GSDMC relative to aged controls. Dexpanthenol increased GSDMD and HSP70 expression, shifted TNF-α toward the young-control profile, and reduced HSP90 expression. Combined administration produced a distinct transcriptional profile characterized by pronounced increases in GSDMD, TNF-α, IL-1β, and HSP70 relative to aged controls. These findings indicate that propolis and dexpanthenol are associated with distinct, gene-specific transcriptional responses in the naturally aged brain rather than uniform changes across the inflammatory and gasdermin-related genes examined. Because the analyses were restricted to mRNA expression, the observed gasdermin transcriptional changes do not establish functional pyroptosis. The prominent HSP70 response further suggests that cellular stress adaptation and proteostasis may represent important components of these treatment-associated molecular changes.PMID:42993343 | DOI:10.1007/s10522-026-10539-5