Fuente: PubMed "honey" Zhongguo Zhong Yao Za Zhi. 2025 Sep;50(17):4870-4879. doi: 10.19540/j.cnki.cjcmm.20250704.402.ABSTRACTThis study aimed to investigate the blood biomarkers for a rat model of spleen-stomach dampness-heat syndrome(SSDHS) treated by Lianpu Drink and unravel the underlying material basis based on the strategy of chinmedomics. Firstly, the rat model of SSDHS was established by a combined method of internal induction(200 g·L~(-1) honey water + wine + lard) + external exposure(artificial climate box). Metabolomics based on ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF-MS) was employed to identify the blood biomarkers. The efficacy of Lianpu Drink in treating SSDHS was evaluated by observation of the general conditions of the rats and examination of the pathological changes in the gastrointestinal tissue. In addition, metabolomics was employed to unravel the endogenous small-molecule metabolites regulated by Lianpu Drink. A total of 32 blood biomarkers of SSDHS model rats were identified. Lianpu Drink significantly reduced the rectal temperature and alleviated the pathological changes in the gastrointestinal tissue of the model rats. 12-Ketodeoxycholic acid, glycodesoxycholic acid, oleoylcarnitine, lysophosphatidylcholine(LysoPC) P-18:0, LysoPC 20:1 sn1, monoacylglycerol(0:0/22:6/0:0), and 3β,16α-dihydroxyandrostenone sulfate were identified as the key callback metabolites in the Lianpu Drink treatment of SSDHS. UPLC-Q-TOF-MS-based serum pharmacochemistry of traditional Chinese medicine was adopted to investigate the in vivo constituents of Lianpu Drink under observable therapeutic effects and a total of 12 in vivo constituents of Lianpu Drink were identified. Molecular docking was adopted to predict the correlation between in vivo constituents and key metabolic enzymes. The results showed that coptisine, berberine, epiberberine, jatrorrhizine, palmatine, shanzhiside, phellodendrine, gentiobioside, magnoloside F, and magnolol had good binding affinity with the key enzymes of callback metabolites, including choloylglycine hydrolase(CGH), 7α-dehydroxylase(7α-DH), phospholipase A2(PLA2), carnitine palmityl transferase Ⅰ(CPT-Ⅰ), sulfotransferase 2A1(SULT2A1), and sulfatase(SULF). In conclusion, the results suggest that Lianpu Drink may treat SSDHS by regulating the enterohepatic circulation of bile acids, inflammatory responses, and energy metabolism. The material basis of Lianpu Drink in the treatment of SSDHS may be coptisine, berberine, epiberberine, jatrorrhizine, palmatine, shanzhiside, phellodendrine, gentiobioside, magnoloside F, and magnolol. This study provides a theoretical basis for the optimization of preparation process, the establishment of quality standards, and the development of innovative drugs for Lianpu Drink.PMID:41507751 | DOI:10.19540/j.cnki.cjcmm.20250704.402