Fuente: PubMed "apis cerana" J Invertebr Pathol. 2025 Oct 29;214:108485. doi: 10.1016/j.jip.2025.108485. Online ahead of print.ABSTRACTMelissococcus plutonius, the agent of European foulbrood (EFB), has been well studied in Apis mellifera but its epidemiology in Apis cerana remains less understood. We surveyed 37 apiaries across Guangxi, China, and detected M. plutonius in 32.4%, 10.8%, and 27.0% of larvae, adult bees, and honey samples, respectively, all originating from asymptomatic colonies. Duplex PCR revealed frequent co-detection of typical and atypical strains. Viable isolates were recovered from 58.3% of PCR-positive larval samples. Multi-locus sequence typing (MLST) showed six isolates (T1, X16, L20, Q21, Q22, L32) clustering with the atypical reference strain DAT561, and one isolate (P6) clustering with the typical reference strain ATCC 35311. Our findings highlight the high prevalence and strain diversity of M. plutonius in A. cerana in subtropical Guangxi and emphasize the need for region-specific surveillance strategies.PMID:41173176 | DOI:10.1016/j.jip.2025.108485