Fuente: PubMed "Tobacco production" Pest Manag Sci. 2026 Mar 8. doi: 10.1002/ps.70696. Online ahead of print.ABSTRACTBACKGROUND: Black shank disease caused by Phytophthora nicotianae threatens global tobacco production, while the invasive plant Mikania micrantha is a high-biomass source of antimicrobial secondary metabolites. To promote the resource utilization of invasive plants for green disease management, this study investigated the antifungal activity and underlying mechanisms of mikanolide, a sesquiterpene lactone derived from M. micrantha, against P. nicotianae.RESULTS: Mikanolide inhibited mycelial growth in vitro (EC50 = 6.519-8.954 μg mL-1), outperforming azoxystrobin. Pot trials demonstrated strong in vivo efficacy, reducing disease incidence and index to 33.33% and 11.48%, respectively. Mechanistically, mikanolide disrupted cell membrane integrity and induced oxidative stress by suppressing ATP synthesis and antioxidant enzyme activities. Transcriptomics revealed upregulation of acetyl-CoA carboxylase and ATP-dependent protease La, with downregulation of malate synthase A. Molecular docking confirmed strong binding affinities to ATP synthase (-7.3 kcal mol-1) and succinate dehydrogenase (-8.0 kcal mol-1).CONCLUSION: Mikanolide suppresses P. nicotianae through disruption of membrane integrity, oxidative stress, and energy metabolism. This study provides a theoretical basis for the resource utilization of M. micrantha and the green management of P. nicotianae. © 2026 Society of Chemical Industry.PMID:41796043 | DOI:10.1002/ps.70696