Fuente: PubMed "Tobacco production" Front Plant Sci. 2026 Jun 4;17:1842437. doi: 10.3389/fpls.2026.1842437. eCollection 2026.ABSTRACTINTRODUCTION: The tripartite symbiosis of legume-AMF-rhizobia is widely considered to facilitate nitrogen (N) uptakeby legumes, but its effect on non-legume plants in intercropping systems remains unclear.METHODS: A pot experiment with three root separations (PS, MS, NS) was conducted using ¹⁵N isotope tracing in a soybean/tobacco intercropping system with double inoculation of Claroideoglomus etunicatum (CE) and Bradyrhizobium japonicum 5016 (BJ).RESULTS: Double inoculation (CE+BJ) significantly increased bioaccumulation, N uptake, and N transfer from soybean to tobacco compared to single or no inoculation. Mycorrhizal colonization in soybean increased by up to 45.55% in the NS system. All measured parameters in tobacco were significantly higher in NS than in PS or MS systems.DISCUSSION: The legume-AMF-rhizobium triple interaction positively affects N uptake and translocation in tobacco, showing potential for sustainable tobacco production, though the underlying mechanisms require further study.PMID:42328075 | PMC:PMC13277567 | DOI:10.3389/fpls.2026.1842437