Fecha de publicación: 10/12/2021 Fuente: Wipo Tobacco Agriculture The invention discloses a tobacco NtCLC-f gene mutant and a molecular identification method and application thereof, the tobacco NtCLC-f gene mutant is Ntclc-f, C at the 1003rd site of a tobacco NtCLC-f gene is mutated into T, a termination codon is formed, and the gene is terminated in advance; and the nucleotide sequence of the gene mutant Ntclc-f is as shown in SEQ ID NO.2. According to the tobacco NtCLC-f gene mutant, the molecular identification method and the application, the tobacco NtCLC-f gene mutant, the molecular identification method and the application are provided for the first time through systematic research, according to a tobacco plant obtained through the NtCLC-f gene mutant Ntclc-f capable of reducing the chlorine ion content in the tobacco leaves, the chlorine ion content in the tobacco leaves is reduced by 28.6% compared with a control group, and the chlorine ion content in the tobacco leaves is remarkably reduced.