Fecha de publicación: 28/01/2022 Fuente: Wipo Tobacco Agriculture The invention discloses a tobacco NtCCC1 gene mutant, a molecular identification method and an application, the tobacco NtCCC1 gene mutant is Ntccc1-1, T at the 650th site of a tobacco NtCCC1 gene is mutated into A, a termination codon is formed, and the gene is terminated in advance; and the nucleotide sequence of the gene mutant Ntccc1-1 is as shown in SEQ ID NO. 2. According to the tobacco NtCCC1 gene mutant, the molecular identification method and the application, the tobacco NtCCC1 gene mutant, the molecular identification method and the application are provided for the first time through systematic research, the NtCCC1 gene mutant capable of reducing the chlorine ion content in the tobacco leaves is a tobacco plant obtained through Ntccc1-1, the chlorine ion content in the tobacco leaves is reduced by 57.1% compared with a control group, and the chlorine ion content in the tobacco leaves is remarkably reduced.