Fecha de publicación: 28/06/2022 Fuente: Wipo Tobacco Agriculture The invention discloses recombinant saccharomyces cerevisiae for producing alpha-cubene and application of the recombinant saccharomyces cerevisiae, and belongs to the field of metabolic engineering and bioengineering. According to the recombinant saccharomyces cerevisiae strain for producing alpha-cubene, saccharomyces cerevisiae is taken as a host, alpha-cubene synthase is subjected to heterologous expression, and farnesyl pyrophosphate synthase and isopentenyl pyrophosphate isomerase are subjected to overexpression. The recombinant saccharomyces cerevisiae strain for producing the alpha-cubene is obtained by transferring recombinant plasmids capable of expressing an alpha-cubene synthase gene, a farnesyl pyrophosphate synthase gene and an isopentenyl pyrophosphate isomerase gene into saccharomyces cerevisiae. According to the invention, the exogenous gene is introduced into the saccharomyces cerevisiae to produce the alpha-cubene, and petroleum resources are not required to be used for chemical synthesis, so that the consumption of the petroleum resources and the pollution to the environment are reduced, and the sustainable development is realized. The alpha-cubene can be efficiently produced, and the application of the alpha-cubene in the fields of food, medicine, agriculture, tobacco and the like is promoted.