Fecha de publicación: 30/11/2021 Fuente: Wipo Tobacco Agriculture The invention discloses quinoline alkaloid (I) as well as a preparation method and application thereof. The method comprises the following steps of: by taking cigar tobacco leaves as a raw material, extracting with high-concentration methanol, high-concentration acetone/water or high-concentration ethanol/water, merging extracting solutions, filtering, and concentrating under reduced pressure to obtain extract; packing the extract by using a silica gel dry method and carrying out silica gel column chromatography; carrying out gradient elution by using a chloroform-acetone solution; and further separating and purifying the 7: 3 part of the eluent by using high-pressure liquid chromatography to obtain the required quinoline alkaloid. The invention further discloses application of the compound. Activity tests show that the compound has a good inhibiting effect on the tobacco mosaic virus. The compound disclosed by the invention is novel in structure and relatively good in anti-toxic activity, and can be used as a lead compound for resisting the tobacco mosaic virus for research and development of a tobacco mosaic virus resisting medicinal preparation.