Fecha de publicación: 04/05/2021 Fuente: Wipo Tobacco Agriculture The invention discloses a protein related to Perilla frutescens L. terpene substance biosynthesis, an encoding gene of protein, and application, and is the protein consisting of an amino acid sequence disclosed by a sequence 2 in a sequence table, and a gene which encodes the protein related to the Perilla frutescens L. terpene substance biosynthesis, and the encoding sequence of the gene is a DNA molecule disclosed by a sequence 1 in the sequence table. The invention provides a pfGPPS protein and an encoding gene thereof, the encoding gene is imported into tobaccos to obtain a tobacco plant which carries out overexpression on a pfGPPS gene, then, the transgenosis tobacco plant is subjected to pot culture, during a mature period, transgenosis tobacco leaves are harvested, and the content of terpene substances is detected through GC-MS. Compared with wild tobaccos, the content of the terpene substances in the transgenosis tobacco is obviously improved. Therefore, the pfGPPS gene and the protein encoded by the pfGPPS gene perform an important function in a plant terpene substance biosynthesis process. The pfGPPS gene provided by the invention and the protein encoded by the pfGPPS gene have an important application value in research of improvement of the content of the plant terpene substances.