Fecha de publicación: 27/09/2022 Fuente: Wipo Tobacco Agriculture The invention belongs to the technical field of plant genetic engineering, and provides a peanut NBS-LRR coding gene AhRRS2 and application thereof in bacterial wilt resistance of plants. The length of the CDS sequence of the AhRRS2 gene is 2433 bp, and the specific nucleotide sequence of the AhRRS2 gene is as shown in SEQ ID No. 1. A CaMV 35S promoter is constructed through a one-step connection method to drive an over-expression vector of the AhRRS2 gene, agrobacterium tumefaciens-mediated transformation of a tobacco variety Honghua Dajinyuan with bacterial wilt, molecular detection and ralstonia solanacearum inoculation identification are carried out on a transgenic plant, the over-expression of the AhRRS2 gene in the transgenic plant can significantly improve the resistance of the transgenic tobacco to the bacterial wilt, and the application prospect is broad. The result shows that the AhRRS2 may participate in the resistance reaction of the plant to the bacterial wilt, which provides an important gene resource for the bacterial wilt resistant genetic engineering breeding application of the plant.