Fecha de publicación: 28/01/2022 Fuente: Wipo Tobacco Agriculture The invention relates to a multiple RT-PCR method and a kit for simultaneously detecting four viruses, namely TSWV, TZSV, PCSV and INSV. The method and the kit both comprise four groups of primers: PCSV-muF/PCSV-muR, INSV-muF/INSV-muR, TSWV-muF/TSWV-muR and TZSV-muF/TZSV-muR. The method and the kit have the beneficial effects that the primer provided by the invention has strong specificity and high sensitivity, is very efficient in the actual multiplex RT-PCR detection process, and can be used for simultaneously detecting the TSWV, the Tomato zonate spot virus, TZSV, the Impatiens necrotic spot virus (INSV) and the pepper chlorotic spot virus (PCSV).