Fecha de publicación: 02/11/2021 Fuente: Wipo Tobacco Agriculture The invention relates to a method for screening a cinnabar plant based on a CYP82E4 gene expression level. The gene is a CYP82E4 gene. The method comprises the following steps: single plant listing and numbering, leaf sampling, RNA extraction and cDNA synthesis, gene expression level detection, and bagging and selfing seed collection. The method has the beneficial effects that the primer is designed by using CYP82E4 gene transcript sequence information, the amplification efficiency is high, and the specificity is strong.