Fecha de publicación: 11/01/2022 Fuente: Wipo Tobacco Agriculture The invention relates to a method for quantitatively analyzing 11 amide alkaloids in tobacco leaves by gas chromatography-tandem mass spectrometry. The method comprises the following steps: soaking in a sodium hydroxide solution to dissociate the amide alkaloids from a tobacco powder sample matrix, and extracting by methyl tert-butyl ether to transfer a target compound into an ether layer; and concentrating methyl tert-butyl ether extract liquor, performing gas chromatography-tandem mass spectrometry, inputting a chromatographic peak area obtained by an instrument into a corresponding standard calibration curve fitting equation of amide alkaloid to obtain a corresponding target compound concentration, and converting to obtain the content of the corresponding amide alkaloid in tobacco leaves. The method can be used for quantitatively analyzing 11 amide alkaloids at the same time, and has the advantages of simplicity, rapidness, stability and the like. The blank that no method for quantitatively analyzing 11 amide alkaloids in tobacco leaves exists at present is filled, and various applications can be developed on the basis.