Fecha de publicación: 11/02/2022 Fuente: Wipo Tobacco Agriculture The invention relates to a method for quantitatively analyzing 11 amide alkaloids in tobacco leaves by gas chromatography-quadrupole mass spectrometry. The method comprises the following steps: soaking in a sodium hydroxide solution to dissociate the amide alkaloids from a tobacco powder sample matrix, extracting by methyl tert-butyl ether to transfer a target compound into an ether layer; concentrating a methyl tert-butyl ether extracting solution, performing gas chromatography-quadrupole mass spectrometry, inputting a chromatographic peak area obtained by an instrument into a standard calibration curve fitting equation of corresponding amide alkaloid to obtain a corresponding target compound concentration, and converting to obtain the content of the corresponding amide alkaloid in tobacco leaves. The method can be used for quantitatively analyzing 11 amide alkaloids at the same time, and has the advantages of simplicity, rapidness, stability and the like. The blank that no method for quantitatively analyzing 11 amide alkaloids in tobacco leaves exists at present is filled.