Fecha de publicación: 16/09/2022 Fuente: Wipo Tobacco Agriculture A method for improving heat-not-burn cigarette quality through biological enzyme pretreatment of tobacco leaves comprises the following steps: (1) raw material treatment: tobacco stems in leaves of redried flue-cured tobacco are removed, and the leaves are left; (2) preparing an enzyme solution, namely preparing alpha-amylase or cellulase into the enzyme solution; (3) enzyme pretreatment: spraying 30-50mL of enzyme liquid on the surfaces of every 100g of tobacco leaves, putting the tobacco leaves sprayed with the enzyme liquid into an incubator at 45-55 DEG C, and pretreating for 3-5 hours at the humidity of 60-80%; (4) fermentation and alcoholization of the pretreated tobacco leaves: the tobacco leaves subjected to enzyme pretreatment are put into an incubator at the temperature of 35-40 DEG C to be fermented and alcoholized for 6-8 days, the tobacco leaves subjected to biological enzyme pretreatment fermentation and alcoholization are obtained, and the humidity is 60-80%; and (5) cigarette preparation: processing the tobacco leaves subjected to bio-enzyme pretreatment, fermentation and alcoholization according to industrial standards to prepare the heat-not-burn cigarettes. The method has wide significance in retaining aroma substances of the flue-cured tobacco leaves, reducing irritation of the tobacco leaves, improving the cigarette quality, shortening the fermentation period and saving the economic cost.