Fecha de publicación: 16/09/2022 Fuente: Wipo Tobacco Agriculture The invention relates to a method for reducing TSNAs in tobacco leaves by utilizing a bacillus altitudinis J54 strain, which comprises the following steps of: A, crushing the tobacco leaves: grinding dried cinnabar tobacco leaves into powder, and sieving; b, preparing a bacterial solution, namely scribing a bacillus altitudinis J54 strain preserved in a glycerol tube on an LB solid culture medium, then putting the LB solid culture medium into a constant-temperature incubator at 28-30 DEG C for culturing, selecting a single colony, transferring the single colony into an LB liquid culture medium, putting the LB liquid culture medium into a constant-temperature shaking incubator for culturing for 20-50 hours to obtain a fermentation bacterial agent, and then regulating the OD value of the bacterial agent to OD600 = 1.8-2.0 by using sterile deionized water to obtain the bacterial solution; the tobacco leaf powder is added into an inorganic salt basic culture medium to serve as a nutrient source of a strain, after high-pressure steam sterilization, bacterial liquid is added, and the usage amount of the bacterial liquid is 0.8%-1.2% of the total volume of a fermentation system; and culturing for 6-8 days in a constant-temperature shaking incubator at 28-30 DEG C at the rotating speed of 150-160 rpm/min or culturing for 7-21 days in a constant-temperature shaking incubator at 35-37 DEG C at the rotating speed of 150-160 rpm/min. According to the method, the TSNAs of the tobacco leaves are innovatively reduced by inoculating the strains.