Fecha de publicación: 18/02/2022 Fuente: Wipo Tobacco Agriculture The invention provides crDNA, crRNA, a kit and a method for detecting plant tomato spotted wilt virus. The nucleotide sequence of the crDNA is as shown in SEQ ID NO.1; the nucleotide sequence of the crRNA is as shown in SEQ ID NO.2; the kit contains the crDNA or the crRNA, a Cas13a protein and a fluorescent probe. The method comprises the following steps of: extracting total RNA of a plant sample to be detected, and acquiring cDNA through reverse transcription; carrying out amplification by taking the cDNA as a template to obtain a DNA amplification product; and using a detection reaction system composed of the amplification product, crRNA, Cas13a protein, the fluorescent probe and enzyme-free water for detection. The crDNA, crRNA, a kit and a method for detecting plant tomato spotted wilt virus provided by the invention have the characteristics of low cost, repeated detection, simple method, high detection speed, sensitivity (the lowest detection limit reaches 10 copies/uL) and specificity.