The invention discloses a co-dominant SSR molecular marker closely linked with a cis-abnieol gene qAbl and application thereof. The serial numbers of the co-dominant SSR molecular marker closely linked with the cis-abnieol gene qAbl are TMc43307 and TMc43341, and the nucleotide sequences of PCR amplification products of molecular marker are respectively as shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3 and SEQ ID No. 4. The invention relates to an application of a co-dominant SSR molecular marker closely linked with a cis-abnieol gene qAbl, in particular to an application of the co-dominant SSR molecular marker closely linked with the cis-abnieol gene qAbl in detecting whether the cis-abnieol gene qAbl exists in tobacco genome DNA or not. The co-dominant SSR molecular marker closely linked with the cis-abnieol gene qAbl provided by the invention has the characteristics of accuracy, high efficiency, stability, convenience and low cost, so that the molecular marker can be applied to molecular marker-assisted selection of the cis-abnieol gene qAbl in tobacco high-aroma-quality breeding.