Fecha de publicación: 05/02/2021 Fuente: Wipo Tobacco Agriculture The invention discloses bacillus velezensis Z002 as well as an acquisition method and application thereof. The bacillus velezensis Z002 is collected in China Center for Type Culture Collection (CCTCC), and the collection number is CCTCC No: M 2020567. The acquisition method comprises separation, screening and purification processes. Endophytic bacteria are firstly separated from tobacco plant tissue and subjected to dark culture, the endophytic bacteria antagonizing the tobacco mildew pathogen rhizopus oryzae are screened out, and finally purification is carried out to obtain the bacillus velezensis Z002. The application of the bacillus velezensis Z002 comprises fermentation, inoculation and modulation processes. Firstly, strains are cultured to obtain a fermentation inoculant, the fermentation inoculant is sprayed to tobacco petioles through a sprayer, tobacco leaves are put into a curing barn, and the tobacco leaves are cured according to a normal curing process. The strain bacillusvelezensis Z002 can effectively control tobacco mildew in the curing period, and the relative control effect is 80% or above. Secondly, the bacillus velezensis Z002 also has very good bacteriostatic effects on Ralstonia pseudosolanacearum, Phylophthora nicotianae, Botrytis cinerea, Sclerotinia sclerotiorum, Thanatephorus cucumeris, Fusarium oxysporum, Alternaria alternata and the like. The bacillus velezensis Z002 has good control effect and higher application and popularization value.