Fecha de publicación: 19/02/2021 Fuente: Wipo Tobacco Agriculture The invention discloses Bacillus velezensis 05-1205 as well as an acquisition method and application thereof, and belongs to the technical field of microbial application. The Bacillus velezensis 05-1205 is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number of the Bacillus velezensis 05-1205 is CCTCC No: M2020568. The acquisition method comprises processes of separating, screening and purifying, firstly, endophytic bacteria are seperated from healthy tobacco plants, endophytic bacteria antagonizing rhizopus oryzae are screened by taking the tobacco mildew pathogenic bacteria rhizopus oryzae in a baking period as an indicator, and finally, purifying is conducted on a culture medium plate to obtain the Bacillus velezensis 05-1205. The application ofthe bacterial strain comprises application in tobacco mildew control, the bacterial strain Bacillus velezensis 05-1205 can achieve effective control over tobacco mildew in the baking period, and therelative control effect is 70% or above. Secondly, the Bacillus velezensis 05-1205 is wide in antibacterial spectrum, and has a very good antibacterial effect on tobacco bacterial wilt bacteria, botrytis cinerea, sclerotinia sclerotiorum, target leaf spot bacteria, root rot bacteria and alternaria alternata besides pathogenic bacteria of tobacco mildew in the baking period.