Fecha de publicación: 20/03/2018 Fuente: WIPO "tobacco agriculture" The invention discloses a tobacco root-knot nematode disease pathogen detection kit based on a loop-mediated isothermal amplification technology and an application method of the tobacco root-knot nematode disease pathogen detection kit. The tobacco root-knot nematode disease pathogen detection kit based on the loop-mediated isothermal amplification technology comprises a reaction solution I for detecting meloidogyne incognita, a reaction solution A for detecting meloidogyne arenaria, a reaction solution J for detecting meloidogyne javanica, a BstDNA polymerase klenow fragment and a 100*SYBR Green I staining solution. The tobacco root-knot nematode disease pathogen detection kit is based on the loop-mediated isothermal amplification technology, has low requirements on detection conditions,can implement the detection only with a water bath pot or a thermostat and facilitates the popularization and application. Since the kit and the method do not need gel electrophoresis, a detection result can be directly observed by naked eyes, so that the consumed time is short, and the detection can be completed in 1.5h; and the kit comprises an LAMP detection primer for detecting the meloidogyneincognita, the meloidogyne arenaria and the meloidogyne javanica, so that the three common pathogen root-knot nematode of tobacco root-knot nematode disease can be synchronously detected.