Fuente: WIPO "tobacco agriculture" The invention discloses a tobacco recessive PVY (Potato Virus Y) resistance gene eIF4E-1 and application thereof. The tobacco recessive PVY resistance gene eIF4E-1 comprises the DNA (Deoxyribonucleic Acid) fragment of an eIF4E-1 gene coding region, wherein the DNA fragment is obtained by PCR (Polymerase Chain Reaction) amplification of a specific primer to a primer 4E-1F (SEQIDNO.3) and a primer 4E-1R (SEQIDNO.4); a nucleotide sequence SEQIDNO.1 of a tobacco PVY resistance gene eIF4E-1 coding region and the amino acid sequence SEQIDNO.2 of protein coded by the tobacco PVY resistance gene are obtained first; the eIF4E-1 gene is a recessive disease resistance gene; the eIF4E-1 gene of infected tobacco is lost by a conventional breeding means, so that the non-GMO (Genetically Modified Organism) disease resistance tobacco is obtained; an interference vector plasmid is built by using the eIF4E-1 gene and converted to the infected tobacco; expression of the eIF4E-1 gene of the infected tobacco is inhibited, so that genetically-modified tobacco with obviously improved disease resistance is obtained; the gene has an important application prospect in cultivating the PVY viral disease resistance tobacco.