Fecha de publicación: 27/02/2024 Fuente: WIPO "tobacco agriculture" The invention belongs to the technical field of gene engineering, and particularly relates to a tobacco Nt epsilon-LCY1 gene nonsense mutant and application thereof. The invention provides a tobacco Nt epsilon-LCY1 gene mutant, the nucleotide sequence of the tobacco Nt epsilon-LCY1 gene mutant is shown as SEQ ID NO: 2, and the tobacco Nt epsilon-LCY1 gene mutant is obtained by changing the 435th nucleotide of a tobacco Nt epsilon-LCY1 gene with the nucleotide sequence shown as SEQ ID NO: 1 into A from G. The tobacco Nt epsilon-LCY1 gene mutant can significantly increase the content of beta-carotene and xanthophyll in tobacco leaves, and can be used for creating tobacco materials with high carotenoid content.