Fecha de publicación: 18/01/2018 Fuente: WIPO "tobacco agriculture" The invention relates to the isolation and the cloning and breeding application of a tobacco mosaic virus (TMV) resistant N′au gene. The invention discloses the nucleotide sequence of the TMV resistant N′au gene shown as SEQ ID NO.1. The amino acids of polypeptide encoded by the TMV resistant N′au gene are shown as SEQ ID NO.2. A non-transgenic TMV resistant tobacco variety can be obtained by transferring an N′au gene which is contained by germplasm resources into a TMV infected popular tobacco variety by conventional breeding means. The N′au gene of the invention has a homologous sequence with high identity rate of nucleotides in the popular tobacco variety, so that it is easy to obtain a shorter introgression segment single plant carrying the N′au gene by conventional breeding, and thereby to obtain a TMV resistant variety with lower linkage drag. The gene can resist both U1 strain and Cg strain of TMV. The novel antiviral gene N′au of the invention has great application prospect in cultivation of a TMV resistant tobacco.