Fuente: WIPO "tobacco agriculture" The invention discloses a tobacco low temperature stress resistant induced early flowering gene NtMYB15 and a cloning method and applications thereof. The nucleotide sequence of the gene NtMYB15 is represented by SEQ ID No.1. The nucleotide sequence of the encoding protein is represented by SEQ ID No.2. By over expressing the gene NtMYB15, the low temperature resistant performance of tobacco can be enhanced, and the gene NtMYB15 has a wide application prospect in the field of controlling the flowering period of tobacco. The provided gene can control related proteins and encoding genes thereof in the flowering period so as to provide gene and technical support for optimizing the breeding of crops especially tobacco in the flowering period.