Fecha de publicación: 12/10/2016 Fuente: WIPO "tobacco agriculture" The invention discloses a tobacco haploid derived from a female parent and a breeding method of the tobacco haploid. The method includes the steps of 1), using hybrid species F1 hybridized by Coker176 and Coker371 Gold as the female parent; 2), hybridizing the hybrid species F1 and African tobacco N. africana serving as the male parent when the difference between the corolla length and the sepal length of the hybrid species F1 is 2.0-2.5cm, sleeving pollinated stigma by the aid of paper tubes after pollination, harvesting mature hybrid seeds, threshing and selecting the seeds and sowing the selected seeds; 3), acquiring plant leaves with the phenotype judged as haploid when each tobacco seedling sowed in the step 2 grows two true leaves, and analyzing the ploidy of the plant leaves by the aid of flow cytometry to obtain the haploid derived from the female parent. The tobacco haploid and the method have the advantages that the method is easy to operate and implement, good in haploid induction effect, high in induction efficiency, fast and accurate in identification, and significant to tobacco haploid breeding.