Fuente: WIPO "tobacco agriculture" The invention discloses a tobacco genome molecular marker probe and sequence collective group as well as an acquiring method and an application thereof. The probe and primer set has any nucleotide sequence as shown in all or partial No9-25. The new probe and primer sequence sets are from a series of new minitype reverse repetitive transposable elements separated and identified by the inventor of the invention. The probe and primer are acquired through a sequence group and independent sequence and through new sequence selection and identification, and can be applied to the following fields: 1, tobacco molecular marker auxiliary breeding, and selecting and breeding of new breeds of traditional tobacco to fasten the process; 2, construction of tobacco genetic spectrum and identification of tobacco DNA fingerprint; 3, important functional gene positioning and cloning in tobacco genome; 4, early-stage identification and selection of important agronomic character of tobacco; 5, genetic relationship identification of different tobacco breeds or strain materials; and 6, true-false identification of tobacco products (including cigarettes).