Fuente: WIPO "tobacco agriculture" The invention discloses a tobacco drought responsive gene NtTIFY as well as a cloning method and application thereof. The gene NtTIFY is capable of coding a tobacco responsive polypeptide, wherein an amino acid sequence included in the polypeptide is as shown in SEQ ID: No.2; and a nucleotide sequence included in the gene NtTIFY is as shown in SEQ ID: No.1. The adaptability of tobacco on drought stress can be improved by inhibiting the expression of the gene. Therefore, the gene NtTIFY has wide application prospects in the field of tobacco drought resistance.