Fuente: WIPO "tobacco agriculture" The invention discloses a tobacco cadmium transporter gene NtHMA4 as well as a cloning method and application thereof. A nucleotide sequence of the tobacco cadmium transporter gene NtHMA4 is as shown in SEQ ID:No.1, and a coded amino acid sequence is as shown in SEQ ID: No.2. The invention further discloses the cloning method of the tobacco cadmium transporter gene NtHMA4. The cloning method comprises the specific steps: A, determining the sequence of the NtHMA4 gene; B, extracting tobacco RNA and obtaining a first chain cDNA by reverse transcription; C, designing and synthesizing a specific primer according to the NtHMA4 gene sequence, and carrying out PCR amplification by taking cDNA as a template; D, recycling and purifying a PCR product; E, connecting a purified product with a carrier, and transforming a competent cell; F, screening positive clone, and carrying out PCR amplification and sequencing onto the positive clone. By regulating expression of the tobacco cadmium transporter gene NtHMA4, transporting rate of cadmium from the tobacco root to leaves can be lowered, so that cadmium content of the tobacco leaves is lowered, and a gene resource is provided for producing green high-quality tobacco leaves.