Fecha de publicación: 13/08/2014 Fuente: WIPO "tobacco agriculture" The invention discloses a tobacco cadmium transporter gene NtHMA2, and a cloning method and application thereof. The nucleotide sequence of the tobacco cadmium transporter gene NtHMA2 is disclosed as SEQ ID:No.1, and the coded amino acid sequence is disclosed as SEQ ID:No.2. The invention also discloses a cloning method of the tobacco cadmium transporter gene NtHMA2, which comprises the following steps: A. determining the NtHMA2 gene sequence; B. extracting tobacco RNA (ribonucleic acid), and carrying out reverse transcription to obtain a first chain cDNA (complementary deoxyribonucleic acid); C. designing and synthesizing specific primers according to the NtHMA2 gene sequence, and carrying out PCR (polymerase chain reaction) amplification by using the cDNA as the template; D recycling and purifying the PCR product; E. connecting the purified product with the vector, and transforming competent cells; and F. screening positive clone, carrying out PCR amplification on the positive clone, and sequencing. By regulating the expression of the tobacco cadmium transporter gene NtHMA2, the method can lower the operation rate of cadmium being transported from the tobacco root to leaves, thereby lowering the chromium content of the tobacco and providing a gene resource for green high-quality tobacco.