Fecha de publicación: 11/07/2012 Fuente: WIPO "tobacco agriculture" The invention discloses a tobacco black shank pathogen liquid-solid composite system culturing method and an application of cultures. Solids obtained by culturing through the method can be applied to artificial inoculums of the tobacco black shank and can also be used for preparing infected soil additives with tobacco black shank pathogens to conveniently determine the resistance of the black shank by the kind of tobaccos and screen medicines, biocontrol bacteria or natural products for controlling the tobacco black shank. The culturing method comprises the following steps: 1, carrying out pathogen separation: acquiring infected strains from an infected field, separating, purifying, and determining the pathogenicity to obtain the pathogens; 2, carrying out plat culturing: inoculating the pathogens onto a medium flat, and culturing for 5-7 days at 24-30DEG C; 3, carrying out liquid culturing: inoculating flat mycelia into a culturing solution, and oscillate-culturing for 5-7 days under conditions that the temperature is 24-30DEG C and the oscillation speed is 150p/min; and 4, carrying out solid culturing: inoculating liquid mycelia into a solid medium, and culturing for 8-12 days at 24-30DEG C. The method has the advantages of large inoculation amount, short culturing period, difficult pollution and the like.