Fecha de publicación: 25/01/2017 Fuente: WIPO "tobacco agriculture" The invention discloses a tobacco axillary bud growth regulation gene NtBRC1 and a cloning method and application thereof. The tobacco axillary bud growth regulation gene NtBRC1 has a nucleotide sequence shown as SEQ ID: No.1 and an encoded amino acid sequence shown as SEQ ID: No.2. The invention also discloses a cloning method of the tobacco axillary bud growth regulation gene NtBRC1. The method comprises the following specific steps: A, determining a gene sequence of NtBRC1; B, extracting tobacco RNA, and performing reverse transcription to obtain a first strand of cDNA; C, designing and synthesizing a specific primer according to the gene sequence of NtBRC1, taking cDNA as a template, and performing PCR amplification; D, recovering and purifying the PCR product; E, purifying the product and vector connection, and transforming competent cells; and F, screening positive clone, and performing PCR amplification and sequencing on the positive clone. By regulating the expression of the tobacco axillary bud growth regulation gene NtBRC1, the development length of the topped tobacco axillary buds can be reduced, so that the usage of a tobacco bud inhibitor is expected to be reduced, and gene resources are provided for producing green high-quality tobacco leaves and reducing the labor cost.