Fecha de publicación: 23/01/2018 Fuente: WIPO "tobacco agriculture" The invention discloses SSR markers linked with resistance genes Bs_t of physiological races No. 0 and No. 1 of tobacco black shank and applications thereof. The SSR markers linked with resistance genes Bs_t of physiological races No. 0 and No. 1 of tobacco black shank are numbered as TM51207 and TM55805, and nucleotide sequences of amplified products of the SSR markers are shown as SEQ ID No. 1,SEQ ID No. 2, SEQ ID No. 3 and SEQ ID No. 4 respectively. The applications refer to applications of the SSR markers linked with resistance genes Bs_t of physiological races No. 0 and No. 1 of tobaccoblack shank in detecting whether resistance genes Bs_t of physiological races No. 0 and No. 1 of tobacco black shank exist in tobacco genomic DNA. The SSR markers disclosed by the invention have the characteristics of being stable, reliable, simple and convenient, rapid and low in cost, and therefore, the molecular markers can be applied as a molecular marker-assisted selection of Bs_t genes in breeding for disease resistance of tobacco black shank (physiological races No. 0 and No. 1).