Fecha de publicación:
20/07/2018
Fuente: WIPO "tobacco agriculture"
The invention discloses a specific primer pair. According to the specific primer pair, an upstream primer sequence is shown as SEQ No. 1 and a downstream primer sequence is shown as SEQ No. 2. The invention further provides application of the primer pair to identification of plant-derived components of cigarettes and an application method of the primer pair. The method comprises the following steps: extracting total DNA (Deoxyribonucleic Acid) of food cigarettes to be detected, carrying out PCR (Polymerase Chain Reaction) amplification, cloning and sequencing, determining the components and the like. By adopting the specific primer pair and a PCR technology, disclosed by the invention, the plant-derived components of the cigarettes can be rapidly, accurately and sensitively detected; the specific primer pair can be applied in the production field.