Fuente: WIPO "tobacco agriculture" The invention discloses simulation cultivation soil with target pathogens, and a production method and application of the simulation cultivation soil with target pathogens. The cultivation soil produced by the method can be used for testing infection resistance of a tobacco variety to Pectobacterium atrosepticum, screening drugs or bio-control agents and natural products for controlling tobacco Pectobacterium atrosepticum. The production method includes: A, breaking pathogen culture solid; B, well mixing the broken pathogen culture solid with culture medium; and C, well mixing the mixture obtained in step B with base soil. Pathogen inoculants in the cultivation soil with target pathogens are stable in quality and controllable in amount, and the cultivation soil can be used for potting and can be subjected to pond application and scattering around tobacco plant roots to serve as artificial pathogenic soil used for field cultivation. By using the pathogen cultivation soil compared with other means, inoculation is simpler, the pathogens live longer, and infection to tobacco plants is more effective.