Fuente: WIPO "tobacco agriculture" The invention discloses a regulatory gene NtMADS1 during a tobacco flowering period as well as a cloning method and application thereof. A sequence of nucleotide contained in the regulatory gene NtMADS1 during the tobacco flowering period is as shown in SEQ ID NO:1; a sequence of amino acid contained in a polypeptide coded by the regulatory gene NtMADS1 during the tobacco flowering period is as shown in SEQ ID NO:2. In tobacco, by reducing the expression of the regulatory gene NtMADS1, the tobacco flowering period can be ahead of time; in addition, by comparing a polypeptide sequence of the regulatory gene NtMADS1, homologous genes having the same functions can be searched, and the functions of the homologous genes can be verified by utilizing a VIGS (Virus-induced Gene Silencing) technology, so that the regulatory gene NtMADS1 has a wide application range; through the regulatory gene NtMADS1 during the tobacco flowering period, genetic and technical supports are provided for optimizing breeding of the tobacco during the flowering period.