Recombinant vector for rapidly obtaining non-GMO genome editing plants and method for using same

Fuente: WIPO "tobacco agriculture"
Provided are a recombinant vector for rapidly obtaining non-GMO genome editing plants and a method for using the same. The recombinant vector comprises an expression element containing PAP1 and NtFT;The original vector is a vector of CRISPR/Cas9 for plant gene editing containing sgRNA gene, Cas9 gene and screening tag gene; The NtFT expression element produces proteins that promote early flowering and the PAP1 expression element produces proteins that promote anthocyanin biosynthesis in plants. The invention can greatly shorten the time for obtaining the gene editing mutation offspring, and screens the offspring plants by plant color, thus not only retaining the offspring of the target gene mutation, but also ensuring that the mutation offspring does not contain the transgenic fragment, and simultaneously can significantly improve the gene editing efficiency. The invention can be used for preparing the gene editing mutation offspring, and can greatly shorten the time for obtaining thegene editing mutation offspring. The method of the invention is simple, fast, cheap and efficient.