Fuente: WIPO "tobacco agriculture" The invention provides a reagent for detecting a tomato zonate spot virus. Optimized gene expression for coding TZSV virus N protein is purified to obtain the TZSV virus N protein, two specific hybridoma cell strains are obtained, and specific anti-TZSV virus monoclonal antibodies can be obtained from the two specific hybridoma cell strains respectively. Test paper for detecting the tomato zonate spot virus is prepared by using the monoclonal antibodies. Experiments prove that the test paper has specificity on TZSV, can achieve + rapid detection of the TZSV virus in samples such as leaves, and is high in sensitivity and convenient to operate.