Fecha de publicación: 28/05/2014 Fuente: WIPO "tobacco agriculture" The invention discloses a rapid extraction method of dry tobacco leaf DNA, comprising steps of material drawing, pretreatment and extraction. The rapid extraction method specifically comprises the following steps: numbering ventilating ziplock bags, picking tender leaves from the bottom of tobacco plants, and putting the tender leaves into the ziplock bags;?putting?the?ziplock bags?into?vacuum pumping pockets, adding?silica gel?into the?pockets and placing the?pockets in a cool place and/or a drying machine/cabinet?until?moisture content?is 1-3%, sealing,?extracting the air?to obtain?dry tobacco leaves;?grinding the obtained?dry tobacco leaves, adding a DNA extraction buffer?and DDT, uniformly mixing, carrying out?thermostatic waterbath, adding?a sodium acetate solution,?uniformly blending?by?oscillation, taking a?supernate?a after centrifugation, taking a supernate?b after second?centrifugation, adding?isopropyl alcohol?of equal volume,?centrifuging and discarding a?supernate?c, dissolving?and washing a?precipitate by adding 70%?alcohol?into the?precipitate,?centrifuging and discarding a supernate?d, adding?absolute ethyl alcohol?into the?precipitate?to wash the?precipitate, drying, dissolving?the precipitate by adding redistilled water into the precipitate, and carrying out electrophoresis detection. According to the invention, the method is simple; the operation is simple and rapid; and DNA extraction efficiency?of a sample is improved.