Fecha de publicación: 30/09/2015 Fuente: WIPO "tobacco agriculture" The embodiment of the invention discloses a phytophthora nicotianae culturing method. The method includes the steps that plate culturing is conducted, phytophthora nicotianae strain is inoculated to a kernel filter liquor culture medium, culturing is conducted for 7 days to 10 days at the temperature of 26 DEG C to 28 DEG C, and sclertium obtained through plate culturing is obtained; solid culturing is conducted, the sclertium obtained through the plate culturing is inoculated into a kernel culturing medium, culturing is conducted for 7 days to 10 days at the temperature of 26 DEG C to 28 DEG C, and strains are obtained; the kernel filter liquor culturing medium is prepared in the mode that shelled wheat is soaked into water with the volume being 1-1.6 times that of the shelled wheat for 8 hours to 12 hours, filtering is conducted through a yarn screen, filtering liquor and filtered shelled wheat are obtained, (17-20) g/L of agar is added to the filtering liquor, and sterilization is conducted; a kernel culturing medium is prepared in the mode that the filtered shelled kernels are mixed with straw grains, the volume ratio between the shelled kernels and the straw grains is (70-90):(10-30), and sterilization is conducted. According to the phytophthora nicotianae culturing method, phytophthora nicotianae culturing is shortened by 5 days to 13 days compared with common phytophthora nicotianae culturing, and the culturing media are simple in formula, convenient to prepare and low in cost.