Fuente: WIPO "tobacco agriculture" The invention discloses a myclobutanil colloidal gold test strip and a preparation method and application thereof. A sample absorbing mat, a conjugate releasing mat and a reaction film of the test strip are sequentially combined on one face of a PVC bottom plate from left to right and from top to bottom. The conjugate releasing mat is overlapped with one end of the reaction film, and the water absorbing mat is combined at the other end of the reaction film. The reaction film covers a detecting line composed of myclobutanil antigens and a quality control line composed of second antibodies, andthe conjugate releasing mat covers a myclobutanil antibody-colloidal gold marker. The preparation method includes the steps of myclobutanil antigen preparation, myclobutanil multi-clonal antibody preparation, colloidal gold preparation, myclobutanil multi-clonal antibody-colloidal gold marker preparation, goat anti-rabbit antibody preparation and colloidal gold test strip assembling. The myclobutanil colloidal gold test strip is applied to detect myclobutanil in plant tissues. The myclobutanil colloidal gold test strip is simple, rapid and accurate in detection, can simultaneously detect a large batch of samples, and meets the requirement for rapid detection of myclobutanil residues in plant tissues such as tobaccos.