Fuente: WIPO "tobacco agriculture" The invention provides molecular markers for assistant selection of a resistance gene (Ph) of tobacco black shank and an application thereof. The molecular markers provided by the invention are TM29 and TM50 which are respectively located on both sides of the gene (Ph) on a 20th gene linkage group and are tightly interlocked with the gene (Ph). The genetic distance from the molecular marker TM29 to the gene (Ph) is 0.091cM and that from the molecular marker TM50 to the gene (Ph) is 0.148cM. The invention further provides a molecular marking method for detecting the resistance gene (Ph) of tobacco black shank by using the molecular markers as well as the application of the molecular markers in assistant breeding of tobacco black shank and positioning of the resistance gene (Ph) of tobacco black shank. The molecular markers for assistant selection provided by the invention is clear in selection target and cost-saving, remarkably improves the selection efficiency, not only provides a resistant resource screening method to breeding of tobacco black shank, but also gets close to the gene (Ph) through a genome walking method, thereby laying a foundation for cloning of the gene (Ph).