Fuente: WIPO "tobacco agriculture" The invention belongs to the field of molecular biology, and discloses a molecular marker for identifying potato virus Y (PVY) resistance of tobacco. The molecular marker contains a sequence shown in SeqIDNo.1. The invention also relates to primers for amplifying the molecular marker, and use of the molecular marker and the primers in disease-resistant resource screening and disease-resistant breeding assisted selection of tobacco. A genome DNA sequence and a tobacco PVY resistant gene are associated by the molecular marker disclosed by the invention, which is beneficial to the building of the assisted breeding selection system of the tobacco molecular marker; the molecular marker is tightly interlocked with the tobacco PVY resistant gene and the interlock distance is 0.99cM. The molecular marker and the molecular marker amplification primers disclosed by the invention can be simply and quickly applied to tobacco breeding practice at high flux.