Fuente: WIPO "tobacco agriculture" The invention discloses a molecular marker for efficiently identifying a tobacco haploid sourced from a female parent and an application thereof. The number of the molecular marker for efficiently identifying the tobacco haploid sourced from the female parent is TM56686 and the nucleotide sequences of the amplification products thereof are respectively shown as SEQ ID No.1 and SEQ ID No.2. The application is that the molecular marker for efficiently identifying the tobacco haploid sourced from the female parent is used for identifying and acquiring the tobacco haploid sourced from the female parent. The molecular marker disclosed by the invention is characterized by stability, reliability, convenience, quickness and low cost, is capable of efficiently and quickly eliminating the diploid and mixoploid plants and is capable of acquiring the tobacco haploid plant sourced from the female parent.