Fuente: WIPO "tobacco agriculture" The invention discloses a method used for simplified identification of tobacco brown spot resistance using artificial disease nursery. The method comprises following steps: disease nursery selection,intensive line-type field overall arrangement, fertilizer controlled cultivation, formation of artificial disease nursery via relatively low concentration large area spraying, moisture retention inducing, and resistance evaluation. According to the method, the characteristics of field tobacco plants, that leaves are susceptible to diseases at maturation stage, and tobacco brown spot is easily caused at an appropriate temperature and high humidity conditions, are taken into consideration, biological zones with appropriate temperature, high humidity, irrigation convenience, and excellent irrigation conditions at tobacco leaf maturation stage are taken as disease nursery; intensive line-type field overall arrangement, fertilizer controlled cultivation, and plant leaf disease susceptibility increasing are adopted, effective inducing of diseases is realized, the accuracy and the reproducibility of tobacco brown spot resistance identification are increased; follow up investigation on the disease infection process of resistant (resistance and moderate resistance) and susceptible reference varieties is taken as a key investigation. Operation is simple; labor intensity is low; both water and labor are saved; cost is reduced; efficiency is high; tobacco brown spot resistant phenotype identification is accurate; and the method is worthy of popularization.