Fecha de publicación: 08/03/2017 Fuente: WIPO "tobacco agriculture" The invention discloses a method of quickly identifying tobacco black shank resistance via an artificial disease nursery. The method comprises steps of manufacturing a black shank inoculants bacteria valley, forming an artificial disease nursery via a layer-cake type inoculating coverage method, maintaining moisture and inducing via an intermittent way and elevating resistance. A feature of susceptibility to diseases of tobacco at a plant field crop land resettling stage and a vigorous-growing period, and biological features of tobacco black shank bacterium surviving, growing and propagating the 5cm depth surface soil are utilized, and the periphery of tobacco plant root parts are inoculated, and a layered-cake type inoculating and covering method of covering a thin layer of organic substance and a thin layer of particular soil to form the artificial disease nursery; and artificial simulation of morning-night intermittent moisture-remaining facilitates shower weather when the black shank disease often happen, and meets high-moisture and ventilating environment requirement for disease occurrence, so the disease can be effectively induced and accuracy and repeatability of tobacco black shank resistance identification can be improved. The method of quickly identifying tobacco black shank resistance via the artificial disease nursery is advantaged by simple operation, water and labor work use reduction and accurate tobacco black shank resistance phenotype identification, thereby being worthy of promotion.