Fuente: WIPO "tobacco agriculture" The invention relates to a method of obtaining a tobacco plant having durable resistance to a potato virus Y. The method comprises the following steps: carrying out knockout (Knockout, KO) on an eIFiso4E-T gene contained in the tobacco plant to obtain an eIFiso4E-TKO mutant, and polymerizing the mutant and PVY wild type virus-resistant va or an eifiso4e-tKO gene to achieve the durable resistance of the tobacco to the PVY. The method has great application prospect for cultivating the anti-PVY tobacco.